proliferation dyes are retained in the cell, even after cell division. Thus,
each generation has half of the fluorescence as the preceding one. The number of
cell divisions can be tracked, based on the continual halving of the
fluorescence in each subsequent generation.
stained with 5 µM Tag-it Violet¢â without stimulation (left) or subsequently
stimulated with 5 µg/mLConA
and 20 µg/mL IL-2 for 4 days (right).
of a cell
detection of injected Tag-it-stained immune cells in tumor
spleen 72 hours after adoptive transfer of
Tag-it Violet¢â-labeled splenocytes
Nucleated cells are stained using 25 µM DRAQ5¢â (Red).
at 40X magnification.
Proliferation and Cell Tracking Dye