ELISpot MAX™ Deluxe Set Human IFN-γ
- Regulatory Status
- RUO
- Other Names
- Interferon gamma, IFN-gamma
- Cat no
- 430124
- Size
- 5 plates
Human peripheral blood mononuclear cells (PBMC) at 104 cells /well were incubated for 20 hours in the absence or presence of 1X Cell Activator without Brefeldin A (Cat. No. 423301), 1X Cell Activator with Brefeldin A (Cat. No. 423303), or Ultra-LEAF™ purified anti-human CD3 (Cat. No. 300331). BioLegend’s Cell Activator cocktail contains PMA and ionomycin at the optimized concentration. Brefeldin A functions to inhibit intracellular protein transport. As a result, it inhibits secretion of cytokines such as IFN-γ. The number of cells secreting human IFN-γ was analyzed by ELISpot.
- Kit Contents
-
- Human IFN-γ ELISpot MAX™ Capture Antibody (200X)
- Human IFN-γ ELISpot MAX™ Detection Antibody (200X)
- Human IFN-γ Standard (used as positive control)
- Avidin-HRP (1000X)
- TMB Membrane Substrate
- Coating Buffer A (5X)
- Assay Diluent B (5X)